Simultaneous determination of melamine, ammelide, ammeline, and cyanuric acid in milk and milk products by gas chromatography-tandem mass spectrometry / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 87-94, 2009.
Artigo
em Inglês
| WPRIM (Pacífico Ocidental)
| ID: wpr-295999
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To develop an analytical method for simultaneously qualitative and quantitative determination of melamine and triazine-related by-products including ammelide, ammeline, and cyanuric acid in milk and milk products by gas chromatography-tandem mass spectrometry (GC-MS/MS).</p><p><b>METHODS</b>Melamine and triazine-related by-products namely ammelide, ammeline and cyanuric acid in the samples were extracted in a solvent mixture of diethylamine, water, and acetonitrile (104050, V/V/V). After centrifugation, an aliquot of the supernatant was evaporated to dryness under a gentle stream of nitrogen gas, and then melamine and triazine-related by-products were derivatized using BSTFA with 1% TMCS. The derivatives of melamine and its analogues were determined by gas chromatography/tandem mass spectrometry using multiple reactional monitoring (MRM) with 2, 6-Diamino-4-chloropyrimidine (DACP) being used as an internal standard.</p><p><b>RESULTS</b>The linear detectable ranges were from 0.004 mg/kg to 1.6 mg/kg for melamine, ammelide, ammeline, and cyanuric acid with a correlation coefficient no less than 0.999. The recovery rates of the four compounds in spiked blank milk powder at concentrations 0.5, 1, 2 mg/kg were between 61.4%-117.2%, and the relative standard deviation was no more than 11.5% (n=6). The detection limits of melamine, ammelide, ammeline and cyanuric acid in milk powder were 0.002 mg/kg with a ratio of signal to noise of 3.</p><p><b>CONCLUSION</b>This GC-MS/MS method for simultaneous determination of melamine, ammelide, ammeline, and cyanuric acid in milk and milk products is sensitive and specific.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Triazinas
/
Dados de Sequência Molecular
/
Contaminação de Alimentos
/
Química
/
Cromatografia Gasosa
/
Sensibilidade e Especificidade
/
Leite
/
Espectrometria de Massas em Tandem
/
Retardadores de Chama
Tipo de estudo:
Estudo diagnóstico
/
Pesquisa qualitativa
Limite:
Animais
Idioma:
Inglês
Revista:
Biomedical and Environmental Sciences
Ano de publicação:
2009
Tipo de documento:
Artigo