Nef mutations in long-term non-progressors from former plasma donors infected with HIV-1 subtype B in China / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 485-491, 2008.
Artigo
em Inglês
| WPRIM (Pacífico Ocidental)
| ID: wpr-296019
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the specific amino acid variation in Nef that may be related to disease progression after infection with HIV-1 subtype B, a predominant strain circulating in China, and to determine whether changes in Nef secondary structure may influence different stages of AIDS development based on the concept that the Nef gene of HIV infection dramatically alter the severity of viral infection and virus replication and disease progression, and that long-term non-progressors (LTNP) of HIV infection are commonly associated with either a deletion of the Nef gene or the defective Nef alleles.</p><p><b>METHODS</b>The study subjects were divided into LTNP1(n=14), LTNP2 (n=16) and slow progressor (SP, n=19) groups for mutational analysis of the Nef sequence. The data were obtained by using Bioedit, MEGA, Anthewin and SAS software.</p><p><b>RESULTS</b>Residues in Nef TA(48/49) and K151 occurred more frequently in the LTNP group while AA(48/49) was more frequently observed in the SP group. Of the differences observed in the secondary structure comparison using Nef consensus sequences of these three groups, one was roughly corresponding to the Nef(48/49) mutation site.</p><p><b>CONCLUSION</b>TA(48/49), K(151), and AA(48/49) in the Nef gene might be associated with the different stages of HIV infection, and there may be a link between the Nef secondary structure and the progression of HIV-1 infection.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fatores de Tempo
/
Virologia
/
Doadores de Sangue
/
Dados de Sequência Molecular
/
Sequência de Bases
/
Produtos do Gene nef
/
Infecções por HIV
/
China
/
Epidemiologia
/
Sequência de Aminoácidos
Limite:
Humanos
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
Biomedical and Environmental Sciences
Ano de publicação:
2008
Tipo de documento:
Artigo