Generation of Japanese Encephalitis Virus-like Particle Vaccine and Preliminary Evaluation of Its Protective Efficiency / 病毒学报
Chinese Journal of Virology
; (6): 150-155, 2016.
Article
em Zh
| WPRIM
| ID: wpr-296204
Biblioteca responsável:
WPRO
ABSTRACT
The cDNA fragment of JEV prME gene was cloned into the baculovirus shuttle vector (bacmid) to construct a recombinant baculovirus vector, defined as AcBac-prME. Then the recombinant baculovirus Ac-prME was obtained by transfecting Sf9 cells with AcBac-prME. Western blot analysis and immunofluorescence results indicated that both prM and E proteins were efficiently expressed in Sf9 cells. Electron microscopy suggested that prME was assembled into JEV-VLPs. To further evaluate the potential of JEV-VLPs as vaccine, the mice were immunized with JEV-VLPs and then challenged with lethal JEV. The results of mice survival and pathological changes demonstrated that the JEV-VLPs performed complete protection against JEV-P3 strain and relieved pathological changes in the mice brain significant. This study suggest that JEV-VLPs would be a potential vaccine for Japanese encephalitis virus.
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Virologia
/
Proteínas do Envelope Viral
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Vacinação
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Encefalite Japonesa
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Vacinas contra Encefalite Japonesa
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Vírus da Encefalite Japonesa (Espécie)
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Alergia e Imunologia
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Vacinas de Partículas Semelhantes a Vírus
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Células Sf9
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Genética
Limite:
Animals
/
Humans
Idioma:
Zh
Revista:
Chinese Journal of Virology
Ano de publicação:
2016
Tipo de documento:
Article