Development of one step RT-PCR technique for detection of H7 subtype avian influenza / 生物工程学报
Chinese Journal of Biotechnology
; (12): 1658-1663, 2009.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-296876
Biblioteca responsável:
WPRO
ABSTRACT
According to 45 hemagglutinin (HA) gene sequences of H7 subtype of avian influenza virus (AIV), a pair of specific oligonucleotide primers was designed. We developed one step RT-PCR for detecting AIV subtype H7. Sensitivity to detection of allantoic fluid by one step RT-PCR reached 10(5.5) EID50/mL and detection of swab samples reached 10(3) EID50/mL. We simultaneity detected the tissue and swab samples infected with H7 subtypes AIV by one step RT-PCR and virus isolation method. The results showed that the sensitivity of the assay gave an excellent correlation with the conventional virus isolation method. H1-H15 subtypes of avian influenza and other avian diseases were detected by the one step RT-PCR. The results showed the assays were high specific, without cross-reaction with other subtypes or other avian diseases. Development of one step RT-PCR will provide effective technical support for the rapid diagnosis and surveillance of molecular epidemiology of AIV subtype H7.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Virologia
/
Aves
/
Sensibilidade e Especificidade
/
Classificação
/
Glicoproteínas de Hemaglutininação de Vírus da Influenza
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Vírus da Influenza A Subtipo H7N1
/
Influenza Aviária
/
Genética
/
Métodos
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2009
Tipo de documento:
Artigo