Inhibitory effect of exogenous insulin-like growth factor binding protein 7 on proliferation of human breast cancer cell line MDA-MB-453 and its mechanism / 生理学报
Acta Physiologica Sinica
; (6): 519-524, 2013.
Article
em Zh
| WPRIM
| ID: wpr-297542
Biblioteca responsável:
WPRO
ABSTRACT
The present study was to investigate the effects of exogenous insulin-like growth factor binding protein 7 (IGFBP7) on the proliferation of human breast cancer cell line MDA-MB-453 and its possible mechanism. By means of MTT method in vitro, the results showed exogenous IGFBP7 inhibited the growth of MDA-MB-453 cells (IC50 of IGFBP7 = 8.49 μg/mL) in time- and concentration-dependent manner. SB203580, p38(MAPK) inhibitor, blocked the anti-proliferative effect of exogenous IGFBP7. The flow cytometry assay showed that exogenous IGFBP7 remarkably induced G0/G1 arrest in MDA-MB-453 cells. The Western blot showed that exogenous IGFBP7 promoted phosphorylation of p38(MAPK), up-regulated expression of p21(CIP1/WAF1), and inhibited phosphorylation of Rb. SB203580 restrained exogenous IGFBP7-induced regulation of p21(CIP1/WAF1) and p-Rb in MDA-MB-453 cells. In conclusion, the present study suggests that exogenous IGFBP7 could activate the p38(MAPK) signaling pathway, upregulate p21(CIP1/WAF1) expression, inhibit phosphorylation of Rb, and finally induce G0/G1 arrest in MDA-MB-453 cells.
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Patologia
/
Farmacologia
/
Fosforilação
/
Piridinas
/
Neoplasias da Mama
/
Somatomedinas
/
Transdução de Sinais
/
Proteínas de Ligação a Fator de Crescimento Semelhante a Insulina
/
Linhagem Celular Tumoral
/
Proteínas Quinases p38 Ativadas por Mitógeno
Limite:
Female
/
Humans
Idioma:
Zh
Revista:
Acta Physiologica Sinica
Ano de publicação:
2013
Tipo de documento:
Article