Detection of transcriptional activities of tumor-specific survivin promoter in human prostatic carcinoma / 中华男科学杂志
National Journal of Andrology
; (12): 502-506, 2007.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-297696
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To clone DNA sequence of the survivin promoter and study is transcriptional activities in human prostate cancer cells and normal Chang liver cells.</p><p><b>METHODS</b>The fragment of the survivin promoter was acquired by PCR amplification and inserted into pPRIME vectors to reconstruct a recombinant plasmid named pPRIME-S1pro and pPRIME-S2pro. Then the reconstructed plasmid was transiently transfected into human prostate cancer cells lines LNCaP and normal Chang liver cells. The transcriptional activities of the survivin promoter in various cells was determined by measuring the expression of green fluorescent protein (GFP).</p><p><b>RESULTS</b>The survivin promoter had transcriptional activities in LNCaP cells and the transcriptional activity of the S2pro was much higher that of the S1pro, reaching a level of 39% of the transcriptional activity of the CMV promoter.</p><p><b>CONCLUSION</b>The survivin promoter cloned in the therapy for prostate cancer.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plasmídeos
/
Neoplasias da Próstata
/
Transfecção
/
Reação em Cadeia da Polimerase
/
Regiões Promotoras Genéticas
/
Linhagem Celular Tumoral
/
Proteínas Inibidoras de Apoptose
/
Genética
/
Metabolismo
/
Proteínas Associadas aos Microtúbulos
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
/
Masculino
Idioma:
Chinês
Revista:
National Journal of Andrology
Ano de publicação:
2007
Tipo de documento:
Artigo