New method of "ingredient difference phonetypical cloning" for functional gene cloning from medicinal plants / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 14-17, 2009.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-298475
Biblioteca responsável:
WPRO
ABSTRACT
This paper introduced a new method of "ingredient difference phonetypical cloning" for functional gene clone of medicinal plants, which might solve the difficulties in isolating genes encoding enzymes for the biosynthesis of secondary metabolites by usual ways. Concepts, mechanisms and methods were systematically introduced and possibility was proved by experiments. The method showed the extra superiority of for the isolation of the genes belonged to unknown metabolic pathway and little information about its sequences. The method provides a new way to isolate functional gene cloning from Chinese herbs and a fundament for the further study on medicinal plant genetic engineering.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fenótipo
/
Plantas Medicinais
/
Fatores de Tempo
/
Medicamentos de Ervas Chinesas
/
Engenharia Genética
/
Reprodutibilidade dos Testes
/
Clonagem Molecular
/
Genes de Plantas
/
Genética
/
Metabolismo
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2009
Tipo de documento:
Artigo