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Effects of chaihu shugan powder on the behavior and expressions of BDNF and TrkB in the hippocampus, amygdala, and the frontal lobe in rat model of depression / 中国中西医结合杂志
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-299001
Biblioteca responsável: WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of Chaihu Shugan Powder (CHSGP) on the behavior and the expressions of brain-derived neurotrophic factor (BDNF) and its receptor tyrosine kinase receptors B (TrkB) in the hippocampus, amygdala, and the frontal lobe of depression model rats.</p><p><b>METHODS</b>Sixty adult Sprague-Dawley rats were randomly divided into 6 groups, i. e., the normal control group (NC), the model control group (MC), the CHSGP group, the disassembly 1 group (CI), the disassembly 2 group (CII), and the Fluoxetine control group (FC), 10 in each group. Except those in the NC, the rest rats were singly housed and exposed on an unpredicted sequence of mild stressor. From the fifteenth day, all rats were administered with equal volume of normal saline (to the NC group and the MC group) and of corresponding medicinal liquid (5.9 g/kg to the CHSGP group, 3.3 g/kg to the CI group, 2.6 g/kg to the CII group, and 1.8 mg/kg to the FC group) by gastrogavage for 2 successive weeks. The rats' body weight, sucrose consumption volume in the sucrose preference test, and times of grooming in the open field test were detected on the 0, 7th, 14th, 21st, 28th day, respectively. The mRNA expressions of BDNF and TrkB in the hippocampus, amygdala, and the frontal lobe were detected by immunohistochemical assay and Real-time fluorescent quantitation PCR.</p><p><b>RESULTS</b>Compared with the NC group, the rats' body weight was put up slowly in the MC group. The scores in the open field test decreased. The times of grooming and sucrose consumption volume were both reduced. The time of staying in central square was postponed. The mRNA expressions of BDNF and TrkB in the hippocampus, amygdala, and the frontal lobe decreased with statistical significance (P<0.05, P<0.01). Compared with the MC group, the behavior indices of rats in the CHSGP, CI, CII, and FC groups were significantly improved. The mRNA expressions of BDNF and TrkB in the hippocampus, amygdala, and the frontal lobe were obviously enhanced with statistical significance (P<0.05, P<0.01).</p><p><b>CONCLUSIONS</b>CHSGP could obviously improve the depressive state of the model rats. Its mechanism might be correlated with increasing the mRNA expressions of BDNF and TrkB in the hippocampus, amygdala, and the frontal lobe.</p>
Assuntos
Texto completo: Disponível Base de dados: WPRIM (Pacífico Ocidental) Assunto principal: Farmacologia / Comportamento Animal / Extratos Vegetais / Ratos Sprague-Dawley / Fator Neurotrófico Derivado do Encéfalo / Receptor trkB / Usos Terapêuticos / Depressão / Tratamento Farmacológico / Lobo Frontal Tipo de estudo: Estudo prognóstico Limite: Animais Idioma: Chinês Revista: Chinese Journal of Integrated Traditional and Western Medicine Ano de publicação: 2011 Tipo de documento: Artigo
Texto completo: Disponível Base de dados: WPRIM (Pacífico Ocidental) Assunto principal: Farmacologia / Comportamento Animal / Extratos Vegetais / Ratos Sprague-Dawley / Fator Neurotrófico Derivado do Encéfalo / Receptor trkB / Usos Terapêuticos / Depressão / Tratamento Farmacológico / Lobo Frontal Tipo de estudo: Estudo prognóstico Limite: Animais Idioma: Chinês Revista: Chinese Journal of Integrated Traditional and Western Medicine Ano de publicação: 2011 Tipo de documento: Artigo
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