Influence of vascular cell co-cultured with osteoblast in vitro on ability of osteoblast / 华西口腔医学杂志

ABSTRACT

<p><b>UNLABELLED</b>OBJECTIVE; The purpose of this study was to observe the influence on proliferation of rabbit osteoblast (ROB) of rabbit vascular endothelial cell(RVEC) co-cultured with rabbit osteoblast (ROB).</p><p><b>METHODS</b>Rabbit osteoblasts and rabbit vascular endothelial cells in ratio of 10, 11, 21, 41 were selected to co-culture. The function of osteoblasts was assessed by alkaline phosphatase (ALP) activity and osteocalcin (OC) assay.</p><p><b>RESULTS</b>There was good compatibility when osteoblasts and vascular endothelial cells were co-cultured. The activity of ALP and OC in the group of ROB and RVEC co-cultured in ratio of 21 was higher than that of the other three groups.</p><p><b>CONCLUSION</b>In direct co-culture system in vitro, RVEC can improve activity of ROB.</p>