A pilot study on the impact of the drug resistance on the radioresistance in human pancreatic cancer cell lines / 中华外科杂志
Chinese Journal of Surgery
; (12): 921-923, 2006.
Article
em Zh
| WPRIM
| ID: wpr-300585
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the impact of the drug resistance on the radioresistance in human pancreatic cancer cell lines.</p><p><b>METHODS</b>Three drug resistant pancreatic cancer cell sublines induced by fluorouracil (5-FU), adriamycin (ADM) and gemcitabine respectively, SW1990/FU, SW1990/ADM and SW1990/Gz, were tested for the cell cycle and radio-sensitivity with flow cytometry and clonogenic assay.</p><p><b>RESULTS</b>Compared with SW1990, the cell cycle assay indicated higher G(0)/G(1) period percentage in SW1990/FU and SW1990/Gz, but the G(2)/M period percentage decreased; SW1990/FU had the same while SW1990/Gz had lower S period percentage. SW1990/ADM almost had a similar cell cycle with SW1990. Clonogenic assay showed both SW1990/FU and SW1990/Gz had greater survival fraction (SF(2)) than SW1990, but SW1990/ADM had seemingly similar SF(2) as SW1990.</p><p><b>CONCLUSION</b>Drug resistant pancreatic cancer cell lines have reduced G(2)/M period percentage and increased radioresistance.</p>
Texto completo:
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Base de dados:
WPRIM
Assunto principal:
Neoplasias Pancreáticas
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Patologia
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Farmacologia
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Tolerância a Radiação
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Doxorrubicina
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Ciclo Celular
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Resistencia a Medicamentos Antineoplásicos
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Linhagem Celular Tumoral
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Desoxicitidina
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Fluoruracila
Limite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Surgery
Ano de publicação:
2006
Tipo de documento:
Article