Effect of combination of docosahexaenoic acid and fluorouracil on human gastric carcinoma cell strain MGC803 / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
; (6): 65-70, 2010.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-301592
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the effect of the combination of docosahexaenoic acid (DHA) and fluorouracil (FU) on human gastric carcinoma cell strain MGC803 in vitro.</p><p><b>METHODS</b>The influences of DHA and FU alone or in combination on cell proliferation was detected using MTT assay. Dose of median (Dm) of drugs (alone or in combination) and the combination index (CI) were calculated using the combination index equation of Chou-Talalay. Cell cycle were examined using flow cytometry. Cell apoptosis was determined by Annexin V-FITC/PI double staining method. The expression of apoptosis-related protein was detected using Western blot.</p><p><b>RESULTS</b>DHA significantly inhibited the growth of MGC803, and low-dose DHA induced the proliferation of human embryonic lung fibroblast (P < 0.05). DHA remarkably strengthened the inhibitive effect of FU on the growth of MGC803, and decreased the Dm of FU by 3.6-2.5 folds (P < 0.05). When the inhibitory ratio reached 30%, the combination of DHA and FU showed synergism (CI < 1) and significant G(0)/G(1) arrest (vs FU, P < 0.05). DHA increased the apoptosis-inducing effect of FU and upregulated the cleaved-caspase-3 expression.</p><p><b>CONCLUSIONS</b>DHA can inhibit the growth of MGC803. When combined with FU, DHA has synergetic effect in inhibiting the proliferation of gastric cancer cells and meanwhile decrease the dose of FU.</p>
Texto completo:
Disponível
Contexto em Saúde:
ODS3 - Meta 3.4 Reduzir as mortes prematuras devido doenças não transmissíveis
Problema de saúde:
Doenças do Sistema Digestório
/
Neoplasia do Estômago
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Farmacologia
/
Neoplasias Gástricas
/
Ciclo Celular
/
Ácidos Docosa-Hexaenoicos
/
Apoptose
/
Linhagem Celular Tumoral
/
Proliferação de Células
/
Sinergismo Farmacológico
/
Caspase 3
Limite:
Humanos
Idioma:
Chinês
Revista:
Acta Academiae Medicinae Sinicae
Ano de publicação:
2010
Tipo de documento:
Artigo