Differential expression of the epithelial membrane protein 1 of laryngeal carcinoma / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
; (6): 47-51, 2003.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-301901
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>In order to explore pathogenic mechanism of laryngeal carcinoma, the involved genes were identified in larynx carcinogenesis by comparing the gene expression profile in matched primary normal epithelial cells and primary laryngeal carcinoma cells from the same patients.</p><p><b>METHODS</b>A cDNA microarray analysis consisting of 11,431 human genes revealed significant changes in the expression of 35 genes, with 8 genes being up-regulated and 27 being down-regulated. The epithelial membrane protein-1 (EMP-1) is one of the down-regulated genes. EMP-1 expression in various kinds of laryngeal carcinoma was determined by semi-quantitative reverse transcriptase polymerase chain reaction (qRT-PCR).</p><p><b>RESULTS</b>The EMP-1 mRNA levels in all laryngeal carcinoma cells was significantly lower than that in the matched primary normal epithelial cells (P < 0.05) and were not correlated to the stage and differentiation of laryngeal carcinoma (P > 0.05).</p><p><b>CONCLUSIONS</b>The EMP-1 expression was correlated to larynx carcinogenesis and may be helpful to elucidate the pathogenic mechanism in laryngeal carcinoma.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Neoplasias Laríngeas
/
Receptores de Superfície Celular
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Reação em Cadeia da Polimerase Via Transcriptase Reversa
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Análise de Sequência com Séries de Oligonucleotídeos
/
Genética
/
Metabolismo
/
Proteínas de Neoplasias
Limite:
Adulto
/
Idoso
/
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Acta Academiae Medicinae Sinicae
Ano de publicação:
2003
Tipo de documento:
Artigo