Effect of catechin on bone marrow cell cycle and gene expression of hematopoietic growth factors / 药学学报
Acta Pharmaceutica Sinica
; (12): 424-428, 2004.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-302794
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>AIM</b>To study the effect of catechin, the active component of Spatholobus suberectus Dunn, on bone marrow cell cycle and the expression of IL-6 and GM-CSF mRNA in spleen cells of normal and marrow-depressed mice in order to clarify the mechanism of hematopoietic-supportive effect of catechin.</p><p><b>METHODS</b>Flow cytometry was adopted to investigate the influence of catechin on bone marrow cell cycle in mice and the expression of IL-6 and GM-CSF mRNA induced by catechin in spleen cells was detected by RT-PCR technique simultaneously.</p><p><b>RESULTS</b>The cell proportion of normal and marrow-depressed mice in G0/G1 phase was reduced significantly, while that in S + G2/M phase increased significantly. Being induced by catechin, IL-6 mRNA and GM-CSF mRNA in spleen cells were markedly up-regulated.</p><p><b>CONCLUSION</b>Catechin (2 g x L(-1), intraperitoneally injected to mice daily immediately after irradiation for 7 consecutive days) was shown to promote the expression of IL-6 mRNA and GM-CSF mRNA in spleen cells of mice, through which it can accelerate bone marrow cells of normal mice into cell cycle and help those of marrow-depressed mice to get out of "G1-phase-block", enter into cell cycle and radically accelerate the proliferation and differentiation of hematopoietic stem cell/hematopoietic progenitor cell (HSC/HPC).</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Plantas Medicinais
/
Baço
/
RNA Mensageiro
/
Células da Medula Óssea
/
Células-Tronco Hematopoéticas
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Catequina
/
Ciclo Celular
/
Química
/
Regulação da Expressão Gênica
Limite:
Animais
Idioma:
Chinês
Revista:
Acta Pharmaceutica Sinica
Ano de publicação:
2004
Tipo de documento:
Artigo