Chromosomal translocation involving USP6 gene in nodular fasciitis / 中华病理学杂志
Chinese Journal of Pathology
; (12): 533-536, 2014.
Article
em Zh
| WPRIM
| ID: wpr-304458
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the frequency of USP6 gene rearrangement in nodular fasciitis (NF) and to evaluate its clinical application.</p><p><b>METHODS</b>Twenty nine cases of previously diagnosed NF were screened for the presence of the USP6 gene rearrangement by interphase fluorescence-in-situ hybridization (FISH) on formalin-fixed paraffin-embedded tissue. Fifteen of these cases, which had available tissue, were also analysed for MYH9-USP6 fusion transcripts by reverse transcription-polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>Twenty four of the 29 cases (83%) were positive for the USP6 gene rearrangement by interphase FISH. The 15 cases with RT-PCR showed the following results: 11 positive, one deletion and three negative for USP6 gene rearrangement. Of these 15 cases, eight (8/15) showed MYH9-USP6 fusion transcript by RT-PCR. Of these eight cases, seven were positive for USP6 gene rearrangement and one showed USP6 deletion by FISH.</p><p><b>CONCLUSIONS</b>USP6 gene rearrangement is a recurrent genetic event in NF. It is a valuable ancillary tool for the pathological diagnosis of these lesions.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Translocação Genética
/
Rearranjo Gênico
/
Proteínas Proto-Oncogênicas
/
Hibridização in Situ Fluorescente
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Ubiquitina Tiolesterase
/
Fasciite
/
Genética
/
Interfase
Limite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Pathology
Ano de publicação:
2014
Tipo de documento:
Article