Enhanced immune response of a novel T-cell immunogen in vaccine for foot-and-mouth disease / 生物工程学报
Chinese Journal of Biotechnology
; (12): 1281-1291, 2011.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-304576
Biblioteca responsável:
WPRO
ABSTRACT
We investigated the enhanced immune response of a recombinant T cell immunogen as an effective cellular immune adjuvant. The T cell immunogen named TI contained several T cell epitopes from the VP1, VP4, 3A and 3D proteins of foot-and-mouth disease virus (FMDV) and two pan-T helper (T(H)) cell sites to broaden the immunogenicity of the protein. Meanwhile, another fusion protein named OA-VP1 was expressed in bacteria, which contained two VP1 proteins of O and Asia1 type FMDV. Mice were vaccinated with commercially inactivated vaccine or OA-VP1 protein with or without the TI immunogen. The results show that mice inoculated with inactivated vaccine or OA-VP1 protein supplemented with TI immunogen produced significantly higher level of neutralizing antibodies (P < 0.01 or P < 0.05) than the mice only inoculated with inactivated vaccine or OA-VP1 protein by microneutralization assay. An obvious increase in T cell number by flow cytometric analysis and significantly higher concentration of IFN-gamma secreted in culture media of spleen lymphocytes were observed in groups supplemented with TI immunogen (P < 0.01). TI immunogen was an effective stimulator for humoral and cellular immunity and could help improve the immunogenicity of inactivated vaccine or protein subunit vaccine.
Texto completo:
Disponível
Contexto em Saúde:
Agenda de Saúde Sustentável para as Américas
/
ODS3 - Saúde e Bem-Estar
Problema de saúde:
Objetivo 10: Doenças transmissíveis
/
Meta 3.3: Acabar com as doenças tropicais negligenciadas e combater as doenças transmissíveis
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Virologia
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Vacinas Virais
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Adjuvantes Imunológicos
/
Imunização
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Epitopos de Linfócito T
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Vírus da Febre Aftosa
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Proteínas do Capsídeo
/
Alergia e Imunologia
/
Febre Aftosa
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2011
Tipo de documento:
Artigo