Enhancement effects of hypoxia on invasion and metastasis of K562 cells / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
; (12): 566-570, 2009.
Article
em Zh
| WPRIM
| ID: wpr-304650
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the potential effect of hypoxia on invasion and metastasis of leukemia cell line K562.</p><p><b>METHODS</b>K562 cells were cultured with the conventional method in vitro and treated with 1%, 3% and 5% oxygen for 24 hrs. The normoxic cultured K562 cells were used as the control group. Cell adhesion assay, cell migration assay and cell invasion assay were used to detect the adhesion, migration and invasion abilities of K562 cells. RT-PCR was used to measure the mRNA expression of HIF-1alpha, VEGF, MMP-2 and MMP-9. The protein level of HIF-1alpha was measured by Western blot.</p><p><b>RESULTS</b>Compared with the control group, the 3% and 5% oxygen treatment groups significantly increased the adhesion, migration and invasion abilities of K562 cells (p<0.05 or <0.01), and up-regulated the protein level of HIF-1alpha and the mRNA levels of HIF-1alpha,VEGF, MMP-9 and MMP-2 (p<0.05 or 0.01). However, there were no significant differences in the above indexes between the 1% oxygen treatment and the control groups.</p><p><b>CONCLUSIONS</b>Moderate hypoxia can enhance the abilities of invasion and metastasis of K562 cells, probably by an up-regulation of HIF-1alpha level and VEGF, MMP-2 and MMP-9 mRNA expression.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Hipóxia Celular
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Adesão Celular
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Movimento Celular
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Células K562
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Metaloproteinase 2 da Matriz
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Metaloproteinase 9 da Matriz
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Células NIH 3T3
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Fator A de Crescimento do Endotélio Vascular
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Subunidade alfa do Fator 1 Induzível por Hipóxia
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Genética
Limite:
Animals
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Humans
Idioma:
Zh
Revista:
Chinese Journal of Contemporary Pediatrics
Ano de publicação:
2009
Tipo de documento:
Article