Effects of dexamethasone on CTLA-4 expression and apoptosis in lymphocytes obtained from children with mesangial proliferative nephritis / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
; (12): 957-960, 2009.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-305149
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>The pathogenesis of mesangial proliferative glomerulonephritis (MsPGN) and mechanisms of glucocorticoid (GC) resistance have not been fully identified. Cytotoxic T-lymphocyte antigen-4 (CTLA-4) is an important inhibitor of T-lymphocyte activation. The objective of the study is to investigate the CTLA-4 expression and apoptosis in lymphocytes of children with MsPGN and the effects of dexamethasone (Dex) on the CTLA-4 expression and apoptosis.</p><p><b>METHODS</b>Blood samples were collected from 36 children with MsPGN and 30 healthy children. CTLA-4 expression in in vitro cultured lymphocytes with or without Dex treatment was measured by flow cytometry following direct immune fluorescene. The rate of apoptosis in the lymphocytes was evaluated by annexin V-FITC and propidium iodide staining.</p><p><b>RESULTS</b>The CTLA-4 expression and apoptosis in lymphocytes from children with MsPGN were significantly lower than those in the healthy control children in the absence or presence of Dex treatment (p<0.05). There was a positive correlation between CTLA-4 expression and apoptosis in lymphocytes (p<0.05).</p><p><b>CONCLUSIONS</b>Abnormal CTLA-4 expression may participate in the pathogenesis of MsPGN and be one of mechanisms of GC resistance.</p>
Texto completo:
Disponível
Contexto em Saúde:
ODS3 - Saúde e Bem-Estar
Problema de saúde:
Meta 3.2: Reduzir as mortes de recém nascidos e crianças com menos de 5 anos
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Sangue
/
Dexametasona
/
Linfócitos
/
Glomerulonefrite Membranoproliferativa
/
Antígenos CD
/
Apoptose
/
Tratamento Farmacológico
/
Alergia e Imunologia
/
Antígeno CTLA-4
Limite:
Criança
/
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Chinese Journal of Contemporary Pediatrics
Ano de publicação:
2009
Tipo de documento:
Artigo