Pulchinenoside control MeCP2 expression in FLS from RA model rats / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 4664-4668, 2014.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-305364
Biblioteca responsável:
WPRO
ABSTRACT
The role of pulchinenoside (PULC) in the regulation of MeCP2 expression was investigated in RA model rats. Adjuvant arthritis rats were used as RA model rats, and fibroblast-like synoviocytes (FLS) from the RA model rats were cultured. The effect of 100 mg x kg(-1) PULC gavage treatment on the MeCP2 expression and the effect of MeCP2 siRNA on the expression of SFRP2 and β-catenin were detected by real time qPCR and Western blotting. The role of PULC in the FLS proliferation was detected by MTT. The results showed that the MeCP2 expression was down-regulated, the SFRP2 expression was up-regulated and the FLS proliferation was inhibited in FLS after therapy. MeCP2 siRNA significantly inhibited the MeCP2 expression, up-regulated the SFRP2 expression and inhibited the β-catenin expression in FLS from RA model rats. PULC may increase the SFRP2 expression, inhibit the Wnt signaling and inhibit the FLS proliferation in FLS from the RA model rats by inhibiting the MeCP2 expression.
Texto completo:
Disponível
Contexto em Saúde:
Agenda de Saúde Sustentável para as Américas
Problema de saúde:
Objetivo 9: Redução de doenças não transmissíveis
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Artrite Reumatoide
/
Membrana Sinovial
/
Medicamentos de Ervas Chinesas
/
Regulação da Expressão Gênica
/
Ratos Sprague-Dawley
/
Biologia Celular
/
Modelos Animais de Doenças
/
Tratamento Farmacológico
/
Beta Catenina
/
Proteína 2 de Ligação a Metil-CpG
Limite:
Animais
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2014
Tipo de documento:
Artigo