Effects of SDS, PBS solutions upon fluorescence values of Dermatophagoides pterronyssinus radioallergosorbent test inhibition / 中国医学科学杂志(英文版)
Chinese Medical Sciences Journal
; (4): 27-29, 2005.
Article
em En
| WPRIM
| ID: wpr-305467
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effects of SDS, PBS re-dissolvent solutions on fluorescence values of radioallergosorbent test (RAST) inhibition.</p><p><b>METHODS</b>Dermatophagoides pterronyssinus allergen immunoCAP and UniCAP 100 System were used. The Sera Pool consisted of 20 Dermatophagoides pterronyssinus allergic patients sera, their specific IgE fluorescence values were between 12505 and 24776.</p><p><b>RESULTS</b>Fluorescence value percentages decreased: 62.9%, 54.1%, 43.5%, 6.7%, 3.7%, 2.6%, 2.2%, and 1.4% respectively, when SDS concentrations were at 2%, 1%, 0.5%, 0.25%, 0.1%, 0.05%, 0.025%, and 0.01%. Fluorescence values decreased more than 5% with SDS concentrations equal to 0.25% or higher. PBS in 0.1 and 0.01 mol/L concentrations decreased fluorescence values 2.9% and 0.9% respectively.</p><p><b>CONCLUSIONS</b>SDS is a commonly used surfactants in allergen extract and re-dissolvent prepared allergen precipitation for RAST inhibition. Thus effects of surfactants (e.g. SDS) upon the RAST inhibition tests must be considered when they were used as re-dissolvent agents to improve protein resolution in RAST inhibition.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Farmacologia
/
Fosfatos
/
Dodecilsulfato de Sódio
/
Soluções
/
Tensoativos
/
Sangue
/
Imunoglobulina E
/
Teste de Radioalergoadsorção
/
Alérgenos
/
Dermatophagoides pteronyssinus
Limite:
Humans
Idioma:
En
Revista:
Chinese Medical Sciences Journal
Ano de publicação:
2005
Tipo de documento:
Article