Detection of anti-HEV IgG and analysis of partial HEV RNA sequence / 中华肝脏病杂志
Chinese Journal of Hepatology
; (12): 405-407, 2003.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-305917
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVES</b>To investigate HEV infection in swine and the genotype relationship between swine and human HEV.</p><p><b>METHODS</b>Anti-HEV IgG antibody was detected in the sera of swine using enzyme linked immunoassay (EIA), and HEV RNA was amplified by reverse transcription nested polymerase chain reaction (RT-nPCR). The Vector NTI Suite 7 and TreeView softwares were used for nucleotide sequences phylogenetic analysis of HEV isolated from human and swine.</p><p><b>RESULTS</b>The anti-HEV IgG positive rate was 16.67% (18/108). Among the 18 anti-HEV IgG positive sera, 2 sequences (11.11%, called S18 and S43, respectively) of HEV ORF1 (102-387bp) were amplified, with the identity of 99% between them. They had 76% to 77%, 78%, 76% to 79%, 85% to 86%, 77%, 80%, 79% and 75% - 79% homology at the nucleotide level with human HEV genotypes 1 to 8, respectively. One (S18) of them was also amplified out in ORF2 region (5,994-6 297bp) and showed 76% to 78%, 74%, 74% to 77%, and 85% to 94% identity with human HEV genotypes 1 to 4 at the nucleotide level, respectively.</p><p><b>CONCLUSION</b>HEV sequences isolated from swine belong to human HEV genotype 4.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Suínos
/
Doenças dos Suínos
/
Virologia
/
Sangue
/
Imunoglobulina G
/
RNA Viral
/
Dados de Sequência Molecular
/
Sequência de Bases
/
Química
/
Classificação
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Hepatology
Ano de publicação:
2003
Tipo de documento:
Artigo