Radiosensitivity on colorectal neoplasms by recombinant adenoviral-mediated wild-type p53 gene / 中华外科杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the radiosensitization on the cells of colorectal cancer transfected with recombinant adenovirus vector-mediated wild-type p53.</p><p><b>METHODS</b>SW480 cells transfected by wild-type p53 were treated with 4 Gy and 6 Gy radiation. The expression of recombinant adenovirus vector-mediated wild-type p53 gene was detected by Western blotting. The inhibition rate of SW480 cells was examined by MTT, apoptotic rate by TdT-mediated dUTP nick end labeling (TUNEL) and proliferating cell nuclear antigen (PCNA) by immunohistochemical method.</p><p><b>RESULTS</b>SW480 cells transfected by wild-type p53 were inhibited significantly by 4 Gy and 6 Gy radiation. The level of apoptosis increased and the expression of PCNA decreased.</p><p><b>CONCLUSION</b>Cells of colorectal carcinoma transfected with wild-type p53 increases their radiation sensitivity.</p>