Expression of miR-143 in nasopharyngeal carcinoma cell lines and its effect on cell adhesion ability / 南方医科大学学报
Journal of Southern Medical University
; (12): 582-585, 2013.
Article
em Zh
| WPRIM
| ID: wpr-306508
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To detect miR-143 expression in human nasopharyngeal carcinoma (NPC) cell lines and explore the role of miR-143 in regulating the adhesion ability of NPC cells.</p><p><b>METHODS</b>Fluorescence quantitative RT-PCR was used to detect miR-143 expression levels in 5 NPC cell lines (CEN1, CNE2, HONE1, 5-8F, and 6-10B) and an immortalized human nasopharyngeal epithelial cell line (NP69). The retrovirus plasmid pMSCV-puro-miR-143 was constructed and the packaged retroviruses pMSCV-puro and pMSCV-puro-miR-143 were infected in 5-8F cells to establish a cell line with stable miR-143 overexpression, whose adhesion ability was tested with adhesion assay.</p><p><b>RESULTS</b>The expression of miR-143 was down- regulated in the NPC cell lines, and the highly metastatic NPC cell line 5-8F had a expression of only 3.0% of the control level, as compared with the level of 63.59% in the tumorigenic but nonmetastatic NPC cell line 6-10B. The transfected 5-8F cells showed a 1080-fold increase of miR-143 expression (P<0.05) with a significantly lowered adhesion ability.</p><p><b>CONCLUSION</b>miR-143 plays a role in regulating the invasiveness and metastasis of NPC, and overexpression of miR-143 causes a significant reduction of the adhesion ability of the highly metastatic NPC cell line 5-8F.</p>
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Base de dados:
WPRIM
Assunto principal:
Patologia
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Carcinoma
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Adesão Celular
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Movimento Celular
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Neoplasias Nasofaríngeas
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MicroRNAs
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Linhagem Celular Tumoral
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Metabolismo
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Southern Medical University
Ano de publicação:
2013
Tipo de documento:
Article