Atorvastatin inhibits scavenger receptor A and monocyte chemoattractant protein-1 expressions in foam cell / 中华心血管病杂志
Chinese Journal of Cardiology
; (12): 666-669, 2007.
Article
em Zh
| WPRIM
| ID: wpr-307223
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of atorvastatin on expressions of scavenger receptor A and secretion of monocyte chemoattractant protein-1 (MCP-1) in foam cells.</p><p><b>METHODS</b>THP-1 cells were induced to differentiate into macrophages by PMA and treated with 0.1% BSA (control), ox-LDL (100 mg/L) or ox-LDL plus atorvastatin (5, 10, 20 micromol/L) for 24 hours. MCP-1 concentration in cell substratum was measured by ELISA. Scavenger receptor A expression was observed under fluorescent microscope after incubated with DiI-Ac-LDL. The relationship between concentration of MCP-1 and the activity of scavenger receptor A was also analyzed.</p><p><b>RESULTS</b>Compared to the control cells, MCP-1 concentration in ox-LDL treated cells was significantly increased after 6 hours, peaked at 12 hours and was still significantly increased after 24 hours (all P < 0.05 vs. baseline). The activity of scavenger receptor A was also significantly increased in ox-LDL treated cells (P < 0.01 vs. control). The activity of scavenger receptor A proteins correlated positively to the concentration of MCP-1 in ox-LDL treated cells (r = 0.683, P < 0.01). Atorvastatin significantly attenuated these changes in a dose-dependent manner.</p><p><b>CONCLUSIONS</b>Scavenger receptor A and MCP-1 expressions were significantly increased in the course of monocyte lines THP-1 differentiating into macrophages and foam cells. The anti-atherosclerosis effect of atorvastatin might be partly achieved by inhibiting the secretion of MCP-1 and expression of scavenger receptor A in foam cells.</p>
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Base de dados:
WPRIM
Assunto principal:
Farmacologia
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Pirróis
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Monócitos
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Diferenciação Celular
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Linhagem Celular
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Quimiocina CCL2
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Biologia Celular
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Receptores Depuradores Classe A
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Atorvastatina
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Células Espumosas
Limite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Cardiology
Ano de publicação:
2007
Tipo de documento:
Article