Effects of shenmai injection on expression of TNF-alpha mRNA in peritoneal macrophages of scald mice / 中华医学杂志(英文版)

ABSTRACT

<p><b>OBJECTIVE</b>To explore the effect of shenmai injection (SI) on expression of TNF-alpha mRNA in peritoneal macrophages (pMPhis) of scald mice.</p><p><b>METHODS</b>BALB/c mice were inflicted with 11% of body surface area III degree scald and injected intraperitoneally (ip) with SI daily for 5 days, and expression of TNF-alpha mRNA in pMPhis was determined by semi-quantitative RT-PCR.</p><p><b>RESULTS</b>In scald mice, the expression of TNF-alpha mRNA in pMPhis increased significantly, but it was reduced obviously (P < 0.01) after SI administration, while the livability was increased markedly (P < 0.05).</p><p><b>CONCLUSIONS</b>For scald mice, the cause of death at early stage might be related to the high expression of TNF-alpha mRNA in pMPhis and the use of SI can decrease the death rate.</p>