Effect of bilirubin on expression of toll-like receptor 4 in cord blood monocytes / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
; (12): 153-156, 2011.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-308850
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of different concentrations of bilirubin on expression of toll-like receptor 4 (TLR4) in cord blood monocytes (CBMC).</p><p><b>METHODS</b>Under the sterile condition, umbilical vein blood samples were obtained from normal full-term newborns, and the monocytes were in vitro separated by the method of gelatin/plasma coated flasks. The monocytes were preincubated with various concentrations (0-307.8 μmol/L) of bilirubin dissolved in bovine albumin solution for 1 hr. Bilirubin-treated CBMC were further cultured with LPS (1 μg/mL) to induce cellular activation for 24 hrs, and then the CBMC were collected. The expression of TLR4 in monocytes was measured by indirect immunofluorescence method.</p><p><b>RESULTS</b>Bilirubin at the concentrations of 102.6, 153.9, 220.6 and 307.8 μmol/L inhibited the expression of TLR4 of CBMC. The inhibition effect increased with the increasing concentration of bilirubin.</p><p><b>CONCLUSIONS</b>Bilirubin can inhibit the TLR4 expression of CBNC in a dose-dependent manner.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Bilirrubina
/
Sangue
/
Monócitos
/
Química
/
Relação Dose-Resposta a Droga
/
Receptor 4 Toll-Like
/
Sangue Fetal
Limite:
Humanos
/
Recém-Nascido
Idioma:
Chinês
Revista:
Chinese Journal of Contemporary Pediatrics
Ano de publicação:
2011
Tipo de documento:
Artigo