Protection of Carassius auratus Gibelio against infection by Aeromonas hydrophila using specific immunoglobulins from hen egg yolk / 浙江大学学报(英文版)(B辑:生物医学和生物技术)
Journal of Zhejiang University. Science. B
; (12): 922-928, 2006.
Article
em En
| WPRIM
| ID: wpr-309052
Biblioteca responsável:
WPRO
ABSTRACT
Specific immunoglobulin (IgY) from egg yolk against Aeromonas hydrophila was produced by immunization of White Leghorn hens with formalin-killed whole cells of A. hydrophila. ELISA test using A. hydrophila as the coating antigen revealed that the specific antibody titer started to increase in the egg yolk at the 13th day post-immunization (P/N=2.18), reached the peak at the 56th day (P/N=13.82), and remained at high level until day 133 (P/N=7.03). The antibody was purified by saturated ammonium sulphate with a recovery rate of 63.5%. The specific IgY inhibited the growth of A. hydrophila at a concentration of 1.0 mg/ml during the 18 h incubation. Pre-treatment of polyploid gibel carps Carassius auratus Gibelio with specific IgY had a protection rate of 60% (6/10) against challenge with A. hydrophila, while none of the fishes in the control groups receiving sterile phosphate buffered saline (PBS) or non-specific IgY survived the challenge. Treatment of fishes with the specific IgY 4 h after the challenge also had lower mortality (70%, 7/10), a 30% reduction against the control PBS or non-specific IgY groups (10/10). These results indicate that specific IgY antibodies could be obtained easily from hens immunized with an inactivated A. hydrophila and could provide a novel alternative approach to control of diseases in fishes caused by this organism.
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Farmacologia
/
Fatores de Tempo
/
Imunoglobulinas
/
Carpa Dourada
/
Testes de Sensibilidade Microbiana
/
Química
/
Galinhas
/
Taxa de Sobrevida
/
Infecções por Bactérias Gram-Negativas
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Aeromonas hydrophila
Limite:
Animals
Idioma:
En
Revista:
Journal of Zhejiang University. Science. B
Ano de publicação:
2006
Tipo de documento:
Article