Aberrant expression of sperm protein 17 enhances migration of ovarian cancer cell line HO-8910 / 中华男科学杂志
National Journal of Andrology
; (12): 982-986, 2008.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-309774
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effects of aberrant expression of sperm protein 17 (Sp17) on the migration of the ovarian cancer cell line HO-8910.</p><p><b>METHODS</b>The recombinant plasmid pEGFP-Sp17 containing Sp17 and enhanced green fluorescent protein gene was transfected into the human ovarian cancer cell line HO-8910 with Lipofectamine 2000. The expression of Sp17 was examined by RT-PCR and Western blot, and the cell migratory capability detected by Transwell chamber assays.</p><p><b>RESULTS</b>Sp17 was expressed as a fusion protein with EGFP after transfected. There was a significant difference in the migratory cell number of the transfected and the control cells (156.6 +/- 14.9/HP vs 39.3 +/- 8.53/HP, P < 0.05).</p><p><b>CONCLUSION</b>The aberrant expression of Sp17 greatly enhances the migration of ovarian cancer cells.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Neoplasias Ovarianas
/
Patologia
/
Transfecção
/
Proteínas de Transporte
/
Biomarcadores Tumorais
/
Regulação Neoplásica da Expressão Gênica
/
Movimento Celular
/
Linhagem Celular Tumoral
/
Genética
/
Metabolismo
Limite:
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
National Journal of Andrology
Ano de publicação:
2008
Tipo de documento:
Artigo