Quality evaluation of bletillae rhizoma based on hemostatic biopotency / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 3764-3767, 2014.
Article
em Zh
| WPRIM
| ID: wpr-310993
Biblioteca responsável:
WPRO
ABSTRACT
This dissertation is to determine the biopotency of hemostat which processed in different places by establishing a bioassay method of Bletillae Rhizoma based on the thrombin time. Contrast test is the main methodology. Specifically, the reference substance of Bletillae Rhizoma is determined by comparing with the control substance of vitamin K1 using thrombin time, which is calibrated the Bletillae Rhizoma. The hemostatic biopotency is calculated by using the method of "parallel line assay method based on quantitative responses" (3.3) from different processed products. It indicates that there is a strong linear correlation between Bletillae Rhizoma and control drugs (Y = 66.332-23.913X, R2 = 0.995 3). The hemostatic biopotency of Bletillae Rhizoma from different processed products ranged between 821.93-1 187.53 U x g(-1) shown in the paper, and all of them can meet the requirements of the test. The methodology has an appropriate instrument precision (RSD 3.8%), intermediate precision (RSD 4.6%), repeatability (RSD 3.2%) and stability (RSD 3.7%). Therefore, it can be turned out that the methodology which established in the dissertation is good at determinating the hemostatic biopotency of Bletillae Rhizoma and it is reliable, simple and repeatable.
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Farmacologia
/
Padrões de Referência
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Tempo de Trombina
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Medicamentos de Ervas Chinesas
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Hemostáticos
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Química
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Ratos Sprague-Dawley
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Orchidaceae
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Rizoma
Limite:
Animals
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2014
Tipo de documento:
Article