Antitumor effects of interleukin-18 gene-modified hepatocyte cell line on implanted liver carcinoma / 中华医学杂志(英文版)
Chinese Medical Journal
; (24): 1475-1479, 2003.
Article
em En
| WPRIM
| ID: wpr-311653
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the antitumor effects of intrasplenically transplanted interleukin-18 (IL-18) gene-modified hepatocytes on murine implanted liver carcinoma.</p><p><b>METHODS</b>Embryonic murine hepatocyte cell line (BNL-CL2) was transfected with a recombinant adenovirus encoding IL-18 and used as delivery cells for IL-18 gene transfer. Two cell lines, BNL-LacZ and BNL-CL2, were used as controls. One week after intrasplenic injection of C26 cells (colon carcinoma line), tumor-bearing syngeneic mice underwent the intrasplenic transplantation of IL-18 gene-modified hepatocyte cell line and were divided into treatment group (BNL IL-18) and control groups (BNL-LacZ and BNL-CL2). Two weeks later, the serum levels of IL-18, interferon-gamma (IFN-gamma), tumor necrosis factor-alpha (TNF-alpha) and nitric oxide (NO) in the implanted liver carcinoma-bearing mice were assayed, the cytotoxicity of murine splenic cytotoxic T-lymphocytes (CTLs) was measured, and the morphology of the hepatic tumors was studied to evaluate the antitumor effects of the approach.</p><p><b>RESULTS</b>In the treatment group, the serum levels of IL-18, IFN-gamma, TNF-alpha and NO increased significantly. The splenic CTL activity increased markedly (P < 0.01), accompanied by a substantial decrease in tumor volume and the percentage of tumor area and prolonged survival of liver carcinomo-being mice.</p><p><b>CONCLUSIONS</b>In vivo IL-18 expression by ex vivo manipulated cells with IL-18 recombinant adenovirus is able to exert potent antitumor effects by inducing a predominantly T-cell-helper type 1 (Th1) immune response. Intrasplenic transplantation of adenovirus-mediated IL-18 gene-modified hepatocytes could be used as a targeting treatment for implanted liver carcinoma.</p>
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Base de dados:
WPRIM
Assunto principal:
Terapêutica
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Transfecção
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Terapia Genética
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Linhagem Celular
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Adenoviridae
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Linfócitos T Auxiliares-Indutores
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Técnicas de Transferência de Genes
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Interleucina-18
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Hepatócitos
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Alergia e Imunologia
Limite:
Animals
Idioma:
En
Revista:
Chinese Medical Journal
Ano de publicação:
2003
Tipo de documento:
Article