Total ginsenosides fought against right ventricular hypertrophy through inhibiting calcineurin signal pathway / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
; (12): 93-97, 2015.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-312973
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effect of total ginsenosides (TG) on monocrotaline (MCT) induced right ventricular hypertrophy rats, and to explore its correlation with calcineurin (CaN) pathway.</p><p><b>METHODS</b>Fifty male Sprague Dawley rats were randomly divided into the normal control group, the MCT model group, and the low, middle, high dose TG treatment groups, 10 in each group. All medication was performed by peritoneal injection for 18 days. Right ventricular peak systolic pressure (RVSP), right ventricular hypertrophy index (RVHI), and right ventricular weight/body weight (RVW/BW) were measured. Intracellular free calcium concentrations were measured by Ca2+ fluorescence indicator Fura2/AM. The atrial natriuretic factor (ANF) and CaN mRNA expression of the myocardial tissue were quantitatively analyzed by Real-time PCR. The protein expression of CaN was detected by Western blot.</p><p><b>RESULTS</b>Compared with the MCT model group, preventive treatment of TG at the 3 doses could significantly reduce RVSP, RVHI, RVW/BW, and ANF mRNA expression, and decrease Ca2+ concentration in myocardial cells, CaN mRNA and protein expression in the myocardial tissue.</p><p><b>CONCLUSION</b>TG could obviously improve MCT-induced right ventricular hypertrophy, which was possibly achieved through suppressing MCT-activated CaN signal transduction.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
RNA Mensageiro
/
Transdução de Sinais
/
Monocrotalina
/
Fator Natriurético Atrial
/
Ratos Sprague-Dawley
/
Hipertrofia Ventricular Direita
/
Calcineurina
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Miócitos Cardíacos
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Ginsenosídeos
/
Usos Terapêuticos
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Integrated Traditional and Western Medicine
Ano de publicação:
2015
Tipo de documento:
Artigo