Characteristics of human adrenal microvascular endothelial cells in morphology, phenotypes, and function / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
; (6): 502-506, 2006.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-313744
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the phenotypic and functional characteristics of human adrenal microvascular endothelial cells (AdrEC).</p><p><b>METHODS</b>AdrEC were isolated and purified from a sample of human adrenal tissue by sub-cell clone method. The cells identified by flow cytometry for classical endothelial markers von Willebrand factor (vWF) and CD31, uptake of Dil-labeled acetylated low density lipoprotein (Dil-Ac-LDL), as well as phenotypes. The cell fenestrations were checked by scanning electron microscopy. The expressions of endogenous vascular endothelial growth factor (VEGF) mRNA and protein were detected by reverse transcription-polymerase chain reaction (RT-PCR) and immunocytochemistry. The glucocorticoid-induced cytotoxicities in different organs-derived microvascular endothelial cells were compared.</p><p><b>RESULTS</b>Human AdrEC expressed those classical endothelial markers such as vWF, CD31, and uptake of Dil-Ac-LDL. The phenotypic analysis indicated that alpha-1 proteinase inhibitor, tumor necrosis factor receptor p55, and intercellular adhesion molecule-1 were expressed in human AdrEC. Scanning electron microscopy demonstrated that there were many microvilli and fenestrations on cellular surface. RT-PCR and immunocytochemistry showed that there was expression of endogenous VEGF in AdrEC. In response to glucocorticoid-induced cytotoxicity, microvascular endothelial cells (MVEC) derived from human brain were highly susceptible, MVEC derived from human lung and human liver sinusoidal endothelial cells were sub-sensitive, while AdrEC were highly resistant.</p><p><b>CONCLUSION</b>Human AdrEC are specially differentiated and have characteristics that are different from other organ-derived MVEC in phenotypes and functions.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fenótipo
/
Fisiologia
/
RNA Mensageiro
/
Células Cultivadas
/
Glândulas Suprarrenais
/
Biologia Celular
/
Células Endoteliais
/
Fator A de Crescimento do Endotélio Vascular
/
Genética
Tipo de estudo:
Estudo prognóstico
Limite:
Humanos
Idioma:
Chinês
Revista:
Acta Academiae Medicinae Sinicae
Ano de publicação:
2006
Tipo de documento:
Artigo