Effect of GMCSF-absence on neovascularization during wound healing / 中华整形外科杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the effect of GMCSF-absence on the rate of wound healing and neovascularization during wound repair.</p><p><b>METHODS</b>30 wild type (WT) mice and 30 GMCSF- absence mice (GMCSF-KO) were obtained. They were received full thickness skin wound (0.8 cm x 0.8 cm) in each side of midline after deeply anesthesia. In the different post-injury time points, the wound sites were digitally photographed to calculate the percentage of wound closure by using computer image analyses software. The wound specimens were also obtained dynamically for immunohistological analysis of CD31 at wound site.</p><p><b>RESULTS</b>The analysis of the wound closure showed that wound healing in GMCSF-KO mice was delayed significantly comparing with that in WT mice from the day 3 post-wounding. At days 7 and 10 after wounding significantly more numbers of blood vessels were formed in the WT controls compared to the GMCSF-KO mice.</p><p><b>CONCLUSIONS</b>GMCSF-absence inhibits neovascularization during wound repair and leads to the delay of wound healing.</p>