Study on the involvement of ZO-1 gene in leukemogenesis / 中华血液学杂志
Chinese Journal of Hematology
; (12): 473-476, 2009.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-314459
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore ZO-1 gene expression and methylation in leukemia cells and the involvement of ZO-1 gene in leukemogenesis.</p><p><b>METHODS</b>Restriction landmark genomic scanning (RLGS) was used to identify new leukemia related gene, and methylation specific PCR (MSP) for ZO-1 methylation status. ZO-1 specific siRNA was designed and prepared by in vitro transcription and transfected into K562 cells, the transfected cells were cultured for 48 hours before harvesting. The effect of ZO-1 siRNA was monitored by Northern blot. Cellular proliferation capacity was assayed by CCK-8, cell apoptosis by Annexin V-fluorescence in isothiocyanate (FITC) assay, and cell cycle by phosphatidylinositol (PI).</p><p><b>RESULTS</b>The intensified spots in RLGS gel were subjected to bioinformatics analysis and one of the candidate spots was proved to be ZO-1 gene. In fresh leukemia cells, Molt4 cells and HL-60 cells, ZO-1 was hypermethylated, causing it reduced or silenced. ZO-1 gene was highly expressed with no methylation in normal peripheral blood MNC and K562 cells. There was a good correlation between promoter methylation and the gene silence. The silenced gene can be re-activated by demethylation treatment with 5-AZA-dC in most leukemia cell lines. RNA interference for ZO-1 gene in K562 cells did not interfere with cell proliferation, cell cycle and apoptosis.</p><p><b>CONCLUSION</b>ZO-1 gene methylation might be involved in the tumorigenesis of acute leukemia.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Fosfoproteínas
/
Leucemia
/
Células HL-60
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Metilação de DNA
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Células K562
/
Proteína da Zônula de Oclusão-1
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Genética
/
Proteínas de Membrana
/
Metabolismo
Limite:
Animais
/
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Hematology
Ano de publicação:
2009
Tipo de documento:
Artigo