Screening of dengue II virus-binding molecules from Aedes albopictus C6/36 cells / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To screen the molecules binding dengue II virus expressed in Aedes albopictus C6/36 cells and characterize their biological functions.</p><p><b>METHODS</b>Aedes albopictus C6/36 cells were infected with dengue II virus, and the virus were collected and purified. The total and membrane proteins of C6/36 cells were extracted and analyzed using 12% SDS-polyacrylamide gel (PAGE). After electrophoresis, the proteins were transferred to a nitrocellulose membrane, and virus overlay protein-binding assay (VOPBA) was carried out using an anti-dengue virus 1-4 monoclonal antibody.</p><p><b>RESULTS</b>Two specific bands of 67 000 and 30 000 occurred after VOPBA of the proteins from the cells incubated with the virus, while the negative control group did not show these specific bands.</p><p><b>CONCLUSION</b>Two putative dengue virus receptor molecules of 67 000 and 30000 have been obtained from C6/36 cells using VOPBA, and their functional identification is in progress.</p>