Protein kinase C mediates thrombin-induced monocyte chemoattractant protein-1 release from human lung fibroblasts / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the role of protein kinase C (PKC) in thrombin-induced monocyte chemoattractant protein-1(MCP-1) release by human lung fibroblasts (HLF-1).</p><p><b>METHODS</b>Cultured human lung fibroblasts HLF-1 were incubated with different concentrations of PKC inhibitors before by thrombin stimulation. MCP-l protein levels in the supernatants were assessed using ELISA, and MCP-1 mRNA levels in the cell lysate were measured by quantitative real-time PCR.</p><p><b>RESULTS</b>The broad spectrum PKC inhibitors bisindolylmaleimide I and RO-31-8220 obviously inhibited thrombin-induced MCP-l mRNA and protein expressions in HLF-1 cells, whereas Ca(2+)-dependent PKC inhibitor Go 6976 had no such effects.</p><p><b>CONCLUSION</b>Ca(2+)-independent PKC mediates thrombin-induced MCP-1 release in cultured HLF-1 cells.</p>