An improved method for primary culture of mouse pulmonary microvascular endothelial cells / 南方医科大学学报
Journal of Southern Medical University
; (12): 1151-1153, 2012.
Article
em Zh
| WPRIM
| ID: wpr-315515
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish an improved method for culturing primary mouse pulmonary microvascular endothelial cells (PMVECs).</p><p><b>METHODS</b>An improved tissue block adherent culture method was used to isolate and culture the PMVECs from C57 mice. The cultured cells were identified by factor VIII-related antigen and CD31 antigen, and the growth of cells cultured using the improved method and the conventional method was compared.</p><p><b>RESULTS</b>The cultured primary pulmonary microvascular endothelial cells showed a short fusiform or round morphology, and the cell monolayer displayed a cobble stone-like appearance. The cultured cells were positive for VIII-related antigen and CD31 antigen. The cell growth was accelerated in the cell cultures with the improved method compared with that in conventional cell cultures.</p><p><b>CONCLUSION</b>The improved culture method allows more efficient acquisition of primary mouse PMVECs of a greater purity.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Endotélio Vascular
/
Células Cultivadas
/
Biologia Celular
/
Células Endoteliais
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Cultura Primária de Células
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Pulmão
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Métodos
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Camundongos Endogâmicos C57BL
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
Zh
Revista:
Journal of Southern Medical University
Ano de publicação:
2012
Tipo de documento:
Article