A novel method for studying nuclear localization signal-mediated nuclear translocation / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To establish a new method for studying the mechanism of nuclear localization signal (NLS)-mediated nuclear translocation in living cells.</p><p><b>METHODS</b>The cells were treated with 67 mg/L 3-[(3-Cholamidopropyl)dimethylammonio]propanesulfonate (CHAPS), followed by incubation with 1 g/L wheat germ agglutinin (WGA), and their effects on interferon- γ (IFN-γ)-induced nuclear translocation of signal transducer and activator of transcription 1 (STAT1) were observed.</p><p><b>RESULTS</b>Treatment with CHAPS alone had no effect on IFN-γ-induced nuclear translocation of STAT1, while this process was blocked by further WGA incubation.</p><p><b>CONCLUSION</b>We established a new, simple but effective method for studying the mechanism of NLS-mediated nuclear translocation in living cells by perforating the cell membrane with CHAPS treatment.</p>