Influence of electromagnetic radiation on raf kinase inhibitor protein and its related proteins of hippocampus / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
; (12): 533-537, 2008.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-315710
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the development of changes for Raf kinase inhibitor protein (RKIP) and its mRNA in rats hippocampus after electromagnetic radiation.</p><p><b>METHODS</b>Rats were exposed to X-band high power microwave (X-HPM), S-band high power microwave (S-HPM) and electromagnetic pulse (EMP) radiation source respectively. The animal model of electromagnetic radiation was established. Western blot was used to detect the expression of RKIP, and RT-PCR was applied to detect the expression of RKIP mRNA. The interaction of RKIP and Raf-1 was measured with co-immunoprecipitation method, and the expression of cerebral choline acetyltransferase (CHAT) was measured by immunohistochemistry.</p><p><b>RESULTS</b>The expression of RKIP significantly down-regulated at 6 h after radiation, and recovered at 1 d in group EMP, but the down-regulation continued during 1 approximately 7 d after radiation in the two microwave groups. The expression of RKIP mRNA changed wavily during 6 h approximately 7 d after radiation, which showed down-regulation at 6 h, and up-regulation at 3 d. The interaction of RKIP and Raf-1 decreased during 6 h approximately 7 d after radiation, most significantly at 7 d, and the two microwave groups were more significant. The expression of CHAT decreased continuously during 6 h approximately 7 d after radiation, and generally recovered on 14 d.</p><p><b>CONCLUSION</b>The down-regulation of RKIP and its related proteins of hippocampus is induced by electromagnetic radiation.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Efeitos da Radiação
/
RNA Mensageiro
/
Ratos Wistar
/
Proteínas Proto-Oncogênicas c-raf
/
MAP Quinase Quinase Quinases
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Proteína de Ligação a Fosfatidiletanolamina
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Radiação Eletromagnética
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Genética
/
Hipocampo
/
Metabolismo
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Ano de publicação:
2008
Tipo de documento:
Artigo