Effect of the peri-implantitis on the biological function of osteoblasts obtained from the mandibles / 华西口腔医学杂志
West China Journal of Stomatology
; (6): 130-133, 2014.
Article
em Zh
| WPRIM
| ID: wpr-315858
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of peri-implantitis inflammatory microenvironment on the biological function of jaw bone osteoblasts.</p><p><b>METHODS</b>Primary mandible osteoblasts from peri-implantitis and normal tissue were isolated and cultured. Third-generation purified osteoblasts were identified and detected. The proliferative activity of osteoblasts was evaluated through MTT assay. Osteocalcin (OCN), Runx2, and collagen I (Col I) mRNA levels were examined by real-time quantitative polymerase chain reaction. OCN protein levels were determined by Western blot.</p><p><b>RESULTS</b>: After 4 d of culture, the proliferative activity of osteoblasts from peri-implantitis became lower than that of normal tissue ( P <0.05). After 7 d of culture, OCN, Runx2, and Col I mRNA expression decreased ( P <0.05). The OCN protein levels also decreased ( P <0.05).</p><p><b>CONCLUSION</b>Peri-implantitis inflammatory microenvironment can decrease the proliferation and differentiation activity of mandible osteoblasts.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Osteoblastos
/
Osso e Ossos
/
RNA Mensageiro
/
Osteocalcina
/
Diferenciação Celular
/
Peri-Implantite
/
Mandíbula
Limite:
Humans
Idioma:
Zh
Revista:
West China Journal of Stomatology
Ano de publicação:
2014
Tipo de documento:
Article