Determination of 3 kinds of components in huanglian jiedu decoction by HPLC under different UV wavelengths / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To determinate 3 kinds of effective components (5 compounds) in Huanglian Jiedu decoction at the same time, namely berberine, palmtine, jatrorrhizine, baicalin and geniposide.</p><p><b>METHOD</b>A HPLC method detected by 3 different UV wavelengths 345 nm for berberine, palmtine and jatrorrhizine, 280 nm for baicalin, 238 nm for geniposide. Diamonsil C18 (4.6 mm x 250 mm, 5 microm) column was used, the mixture of H2O (A) , CH3OH (B) and 0.05% H3PO4 solution (C) as mobile phase in gradient mode. The column temperature was 35 degrees C and the flow rate was 1 mL x min(-1).</p><p><b>RESULT</b>This method was applied to determined 5 compounds in Huanglian Jiedu decoction rapidly and accurately.</p><p><b>CONCLUSION</b>The quality control method is convenient, reasonable and credible for Huanglian Jiedu decoction.</p>