Expression of a begomoviral DNAbeta gene in transgenic Nicotiana plants induced abnormal cell division / 浙江大学学报(英文版)(B辑:生物医学和生物技术)
Journal of Zhejiang University. Science. B
; (12): 83-86, 2005.
Article
em En
| WPRIM
| ID: wpr-316371
Biblioteca responsável:
WPRO
ABSTRACT
An increasing number of monopartite begomoviruses are being identified that a satellite molecule (DNAbeta) is required to induce typical symptoms in host plants. DNAbeta encodes a single gene (termed betaC1) encoded in the complementary-sense. We have produced transgenic Nicotiana benthamiana and N. tabacum plants expressing the betaC1 gene of a DNAbeta associated with Tomato yellow leaf curl China virus (TYLCCNV), under the control of the Cauliflower mosaic virus 35S promoter. Transgenic plants expressing betaC1 showed severe developmental abnormalities in both species. Microscopic analysis of sections of both transgenic and non-transgenic N. tabacum leaves showed abnormal outgrowths of transgenic N. tabacum to be due to disorganized cell division (hyperplasia) of spongy and palisade parenchyma. Immuno-gold labeling of sections with a polyclonal antibody against the betaC1 protein showed that the betaC1 protein accumulated in the nuclei of cells. The possible biological function of the betaC1 protein was discussed.
Texto completo:
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Base de dados:
WPRIM
Assunto principal:
Fisiologia
/
Doenças das Plantas
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Nicotiana
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Proteínas Virais
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Virologia
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Proteínas Recombinantes
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DNA Viral
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Divisão Celular
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Núcleo Celular
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Células Cultivadas
Idioma:
En
Revista:
Journal of Zhejiang University. Science. B
Ano de publicação:
2005
Tipo de documento:
Article