Cellular growth under hydrostatic pressure using bovine aortic EC-SMC co-cultured ePTFE vascular graft / 浙江大学学报(英文版)(B辑:生物医学和生物技术)
Journal of Zhejiang University. Science. B
; (12): 79-82, 2005.
Artigo
em Inglês
| WPRIM (Pacífico Ocidental)
| ID: wpr-316372
Biblioteca responsável:
WPRO
ABSTRACT
High blood pressure (hypertension) is implicated in the development of atherosclerosis. Blood vessels are constantly subjected to stretch due to blood pressure and changes in stretch usually instigate adaptive vascular remodeling, including abnormal growth and proliferation of vascular smooth muscle cells (VSMCs) as well as extracellular matrix (ECM). In this experiment, we used bovine aortic endothelial cells and smooth muscle cells (EC-SMC) co-cultured ePTFE vascular grafts subjected to normal atmospheric pressure (as a control), and 100 mmHg hydrostatic pressure for 7 d. The increase of cell layer thickness was observed. When measured, the cell layer thickness increased by 116.2%. The increase of collagen (Type IV) synthesis was also observed in the immunohistochemistry assay. When stained with toluidine blue, the cells showed metachromatic phenomenon.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fisiologia
/
Politetrafluoretileno
/
Prótese Vascular
/
Células Cultivadas
/
Química
/
Técnicas de Cultura de Células
/
Transplantes
/
Biologia Celular
/
Colágeno Tipo IV
/
Engenharia Tecidual
Limite:
Animais
Idioma:
Inglês
Revista:
Journal of Zhejiang University. Science. B
Ano de publicação:
2005
Tipo de documento:
Artigo