Effect of MT01/PEN complexes on the expression of osteoprotegerin and receptor activator of nuclear factor κB ligand in human osteoblast-like cell line MG63 / 华西口腔医学杂志
West China Journal of Stomatology
; (6): 32-36, 2016.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-317735
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>This study aims to synthesize MTO1 (a kind of oligodeoxynucleotides) and N-isopropylacrylamide-modified polyethylenimines (PEN) complexes (MT01/PEN) as well as to investigate the effect of the complexes on the expression of osteoprotegerin (OPG) and the receptor activator of nuclear factor κB ligand (RANKL) in the human osteoblast-like cell line MG63.</p><p><b>METHODS</b>MG63 cells were transfected by MT01/PEN complexes formed with three different mass ratios (12, 14, 16) of MT01 to PEN. MT01 and MT01-s were used as positive control. Enzyme-linked immunosorbent assay and real-time polymerase chain reaction were performed to estimate the amount of OPG and RANKL released into the culture media and in MG63 at 24, 48, 72 h.</p><p><b>RESULTS</b>MG63 responded to the MT01/PEN complexes by significantly upregulating the OPG on the protein and mRNA levels (P < 0.05). The protein and mRNA levels of RANKL were lower in most of the groups with complexes, and the OPG/RANKL ratio were higher (P < 0.05). MG63 were affected by the MT01/PEN complexes with different mass ratios, particularly when the ratio was 16.</p><p><b>CONCLUSION</b>MT01 can enhance the promotion of ossification by establishing the delivery system with PEN.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Oligodesoxirribonucleotídeos
/
Osteoblastos
/
Polietilenoimina
/
Acrilamidas
/
RNA Mensageiro
/
Ensaio de Imunoadsorção Enzimática
/
Linhagem Celular
/
Ligante RANK
/
Osteoprotegerina
/
Reação em Cadeia da Polimerase em Tempo Real
Limite:
Humanos
Idioma:
Chinês
Revista:
West China Journal of Stomatology
Ano de publicação:
2016
Tipo de documento:
Artigo