Effects of double-stranded RNA poly(I:C) on matrix metalloproteinase mRNA expression in human nasal polyp epithelial cells / 中华耳鼻咽喉头颈外科杂志
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
; (12): 229-232, 2010.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-318227
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>Chronic rhinosinusitis was often exacerbated by viral infection. A disruption of the mechanisms that regulate the activity of matrix metalloproteinases (MMPs) during viral infection was one possible mechanism responsible for the exacerbation. The purpose of study was to achieve a better understanding of MMP expression in nasal epithelial cells after viral infection.</p><p><b>METHODS</b>Human nasal epithelial cells were isolated from nasal polyp specimens obtained during endoscopic endonasal surgery in chronic rhinosinusitis patients. The expression of MMP-2, MMP-9, and tissue inhibitor of metalloproteinase (TIMP)-1 mRNA in primary human nasal polyp epithelial cells after double stranded RNA (ds RNA) stimulation were investigated.</p><p><b>RESULTS</b>Among the genes whose expression was evaluated, only expression of MMP-9 mRNA increased significantly after dsRNA stimulation (22.61 +/- 5.47 fold increase, Z = -2.52, P = 0.012).</p><p><b>CONCLUSIONS</b>The significant up-regulation of MMP-9 mRNA, which was not modulated by TIMP-1, was an additional source of increased proteolytic activity in virus-infected upper airways that might contribute to the exacerbation of chronic rhinosinusitis with nasal polyps.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Sinusite
/
RNA de Cadeia Dupla
/
RNA Mensageiro
/
Regulação Enzimológica da Expressão Gênica
/
Células Cultivadas
/
Pólipos Nasais
/
Poli I-C
/
Inibidor Tecidual de Metaloproteinase-1
/
Metaloproteinase 2 da Matriz
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
Ano de publicação:
2010
Tipo de documento:
Artigo