SCoT differential expression of cold resistance related genes in Dendrobium officinale under low temperature stress / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 511-515, 2013.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-318666
Biblioteca responsável:
WPRO
ABSTRACT
In order to study the molecule mechanism of the differential expression in Dendrobium officinale under low temperature, the high cold resistance germplasms were used for constructing the RNA pools. SCoT markers were used to analyze the different cDNA pools transcribed from the RNA pools. 11 transcripts derived fragments from 500 cDNA amplified bands were amplified by 64 primers, and were sorted out, cloned, sequenced and analyzed. The results showed that cDNA pools with SCoT markers could be used for differential display in D. officinale under low temperature stress. Sequence analysis indicated that the transcripts derived fragments were significantly homologous in nucleotide sequence with membrane-associated proteins, osmotic regulation protein, CBF transcriptional factor, resistance protein. One left gene segments functions were still unknown, which may be related to the cold resistant gene expression in D. officinale.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fisiologia
/
Estresse Fisiológico
/
Cruzamento
/
Marcadores Genéticos
/
Temperatura Baixa
/
Regulação da Expressão Gênica de Plantas
/
Dendrobium
/
Genética
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2013
Tipo de documento:
Artigo