Sequence analysis of a novel HLA-A * 2459 allele / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1090-1092, 2007.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-318783
Biblioteca responsável:
WPRO
ABSTRACT
This study was aimed to investigate the molecular genetics basis of a novel allele HLA-A * 2459 in Chinese population. DNA was extracted from whole blood by PEL-FREEZ DNA extraction kit. The amplification of HLA-A exons 2 - 4 of the proband was preformed by allele specific primer PCR and the amplified product was sequenced bidirectionally with primers. The sequencing results showed HLA-A alleles of the proband as A * 1101 and the novel allele. The sequences of the novel allele have been submitted to GenBank (DQ313255, DQ313256, DQ313257). After Blast HLA analysis, the novel allele showed only one nucleotide differences with HLA-A * 24020101 at nucleotide position 527 T to C in exon 3. This results in an amino acid changes from Val to Ala at codon 152. In conclusion, this allele is a novel one and has been officially named HLA-A * 2459 by the WHO Nomenclature Committee.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Doadores de Sangue
/
Dados de Sequência Molecular
/
Sequência de Bases
/
Antígenos HLA-A
/
China
/
Análise de Sequência de DNA
/
Povo Asiático
/
Alelos
/
Genética
Limite:
Humanos
País/Região como assunto:
Ásia
Idioma:
Chinês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2007
Tipo de documento:
Artigo