Establishment of a culture system of chick embryo for mouse tooth germ development / 华西口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To establish a new culture system for mouse tooth germs in chick embryo.</p><p><b>METHODS</b>The mandibular first molar germ fragments of 15 embryonic days' Kunming mouse embryo were implanted into the lateral mesenchyme of 4-5 days' chick embryo wing buds in ove. Eggs were reincubated and implanted tissues were examined by histochemistry.</p><p><b>RESULTS</b>The cultured tooth germ development continued from cap stage to latest bell stage. The ameloblast and the odontoblast all differentiated maturely and secreted matrix.</p><p><b>CONCLUSION</b>4-5 days' wing buds chick embryo could serve as developing the mouse tooth germs and demonstrate well physiological process of differentiation and morphogenesis.</p>