Construction and identification of lentiviral vector for RNA interference targeting STUB1 gene / 浙江大学学报·医学版

ABSTRACT

<p><b>OBJECTIVE</b>To construct and identification of a lentiviral vector for RNA interference (RNAi) targeting STUB1 gene.</p><p><b>METHODS</b>A pair of complementary small hairpin RNA (shRNA) oligonucleotides targeting STUB1 gene was designed, synthesized and inserted into linearized pMagic 4.0 vector. The recombinant plasmid was identified by double restriction digestion with Age I/EcoR I and DNA sequencing.</p><p><b>RESULT</b>PCR and DNA sequencing showed that the shRNA sequence was successfully inserted into pMagic 4.0 vector. The pMagic 4.0 vector was successfully packaged into lentivirus particles.</p><p><b>CONCLUSION</b>A lentiviral shRNA expression vector and particles targeting STUB1 gene has been successfully constructed for the further study of the STUB1 gene.</p>