Construction of RegIα over-expression vector and its effect on proliferation and apoptosis of gastric cancer cells / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
; (6): 499-505, 2010.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-319869
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To construct RegIα over-expression vector and to evaluate the effect of RegIα on the proliferation and apoptosis of gastric cancer MKN28 cells in vitro.</p><p><b>METHODS</b>Full sequence of RegIα cDNA was amplified from normal gastric tissue samples by RT-PCR and cloned into pIRES2-EGFP vector. RT-PCR and Western blot were performed to detect expression levels of RegIα in MKN28 cells. The effects of over-expression RegIα on cell proliferation was measured by MTT assay and apoptosis was detected by flow cytometry.</p><p><b>RESULT</b>RegIα cDNA over-expression vector of pIRES2-RegIα-EGFP was successfully constructed. The expressions of RegIα in MKN28 cells, including mRNA and protein levels, were significantly increased after stable transfection, which resulted in cell proliferation and anti-apoptotic effect induced by H(2)O(2).</p><p><b>CONCLUSION</b>The over-expression of RegIα can promote cell proliferation and reduce cell apoptosis when induced by H(2)O(2) in gastric cancer cells.</p>
Texto completo:
Disponível
Contexto em Saúde:
ODS3 - Meta 3.4 Reduzir as mortes prematuras devido doenças não transmissíveis
Problema de saúde:
Doenças do Sistema Digestório
/
Neoplasia do Estômago
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Plasmídeos
/
Neoplasias Gástricas
/
Transfecção
/
Apoptose
/
Linhagem Celular Tumoral
/
Proliferação de Células
/
Vetores Genéticos
/
Genética
/
Metabolismo
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Zhejiang University. Medical sciences
Ano de publicação:
2010
Tipo de documento:
Artigo