Identification and sequence analysis of a novel HLA-B*5614 allele / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
; (6): 288-290, 2005.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-321104
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the molecular genetics basis for a novel HLA allele, HLA-B*5614, in Chinese population.</p><p><b>METHODS</b>DNA was extracted from whole blood by salting-out method. The HLA-B exons 2-4 of the proband was amplified and the amplified product was cloned using TOPO cloning sequencing kit to split the two alleles apart. Both strands of exons 2,3 and 4 of chosen colonies were sequencing. The PCR-SSP was performed to confirm the mutations detected by sequencing.</p><p><b>RESULTS</b>The sequencing results showed the HLA-B alleles of the proband as B*1502 and the novel allele. The sequences of the novel allele have been submitted to GenBank (AY601726, AY610727, AY610728). After BLAST analysis, the novel allele differs from B*5608 by a single nucleotide at position 277G-->C in exon 2. This results in an amino acid change from Gly to Arg at codon 93.</p><p><b>CONCLUSION</b>This allele is a novel allele and has been officially named B*5614 by the WHO Nomenclature Committee.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Dados de Sequência Molecular
/
Antígenos HLA-B
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Reação em Cadeia da Polimerase
/
Éxons
/
Análise de Sequência de DNA
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Polimorfismo de Nucleotídeo Único
/
Alelos
/
Genética
/
Métodos
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
Limite:
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Chinese Journal of Medical Genetics
Ano de publicação:
2005
Tipo de documento:
Artigo